Disseminated Acanthamoeba Infection in a Patient with AIDS: Response to 5-Fluorocytosine Therapy Free-living amebas belonging to the genera Naegleria, Acan- thamoeba, and Balamuthia are pathogenic protozoa for humans and animals. Acanthamoeba species and Balamuthia mandrillaris

fertilizer, and manure. Thirty-one strains of Legionella were recovered (table 1). L. longbeachae serogroup 1 was isolated from 8 samples; L. bozemanii serogroup 1, 9; Legionella micdadei, 5; Legionella pneumophila serogroup 4, 1; and L. pneumophila serogroup 12, 1. An unidentifiable Legionella species was isolated from four soil samples; Legionella gormanii, Legionella birminghamensis, and Legionella cincinnatiensis were isolated from one soil sample each. L. longbeachae was isolated from three composted wood soils and five complex soil mixes. Japanese potting soils contain multiple types of Legionella species, most of which have been implicated in human disease. In contrast to the Australian products, the Japanese products are composed of different wood types. Unlike the situation in Australia, L. longbeachae pneumonia and other forms of legionnaires’ disease are rarely diagnosed in Japan, although routine diagnostic testing for the disease is uncommonly undertaken. It is unknown if the Japanese potting soils present any risk for legionnaires’ disease. Further investigation is required to determine the clinical and epidemiological relevance of the presence of Legionella species in potting soils.